Whole campus wastewater surveillance of SARS-CoV-2 for COVID-19 outbreak management.

In this long-term study (eight months), a wastewater-based epidemiology program was initiated as a decision support tool for the detection and containment of COVID-19 spread in the Technion campus. The on-campus students' accommodations (∼3,300 residents) were divided into housing clusters and monitored through wastewater SARS-CoV-2 surveillance in 10 manholes. Results were used to create a 'traffic-light' scheme allowing the Technion's COVID-19 task force to track COVID-19 spatiotemporal spread on the campus, and consequently, contain it before high morbidity levels develop. Of the 523 sewage samples analysed, 87.4% were negative for SARS-CoV-2 while 11.5% were positive, corroborating morbidity information the COVID-19 task force had. For 7.6% of the SARS-CoV-2 positive samples, the task force had no information about positive resident/s. In these events, new cases were identified after the relevant residents were clinically surge tested for COVID-19. Hence, in these instances, wastewater surveillance provided early warning helping to secure the health of the campus residents by minimising COVID-19 spread. The inflammation biomarker ferritin levels in SARS-CoV-2 positive sewage samples were significantly higher than in negative ones. This may indicate that in the future, ferritin (and other biomarkers) concentrations in wastewater could serve as indicators of infectious and inflammatory disease outbreaks.

A continuous active monitoring approach to identify cross-connections between potable water and effluent distribution systems.

A continuous active monitoring approach was developed for identification of cross-connections between potable water supply systems and treated wastewater effluent reuse distribution systems. The approach is based on monitoring the oxidation reduction potential (ORP) at the potable water system while injecting sulfite (a reducing agent) into the effluent line. A sharp decrease in the ORP of the potable water would indicate a cross-connection event. The approach was tested in batch experiments on treated municipal wastewater effluent of varying degree of treatment, and at dilution ratios of up to 1:100 (effluent/potable). The approach was then examined under continuous flow conditions, which simulated cross-connection events at various dilution ratios (up to 1:100). In the continuous runs, differences between the potable water ORP and the effluent-potable water mixture (containing sulfite as sodium bisulfite (SBS)) ORP were 450-630 mV. This suggests high potential for identifying a cross-connection event. Implementation of the approach includes adding sulfite to effluent used for agricultural irrigation; hence, possible effects on soil and on crops were studied in soil columns and pots planted with basil (Ocimum basilicum) as a model plant. No negative effects of sulfite addition to the irrigation effluent were observed in the irrigated soils and plants, and therefore, it could be safely implemented also in agricultural applications.

Reliability of on-site greywater treatment systems in Mediterranean and arid environments - a case study.

On-site greywater (GW) treatment and reuse is gaining popularity. However, a main point of concern is that inadequate treatment of such water may lead to negative environmental and health effects. Maintenance of single-family home GW systems is usually performed by home owners with limited professional support. Therefore, unless GW systems are reliable, environmental and public health might be compromised. This study is aimed at investigating the reliability of on-site recirculated vertical flow constructed wetlands (RVFCW) in 20 single-family homes. In order to ensure reliability, the failure-tree approach was adopted during the design and construction of the systems. The performance of the systems was monitored for 1.5 years, by evaluating treated GW flow and quality, and by recording all malfunctions and maintenance work. Only 39 failures occurred during this period, of which four caused irrigation with impaired quality GW, while the rest led to no irrigation. The mean time between failures (MTBF) was 305 days; two out of the 20 systems suffered from seven malfunctions (each), while nine systems did not fail at all. Thus, it can be postulated that if on-site GW treatment systems are designed with the right controls, and if scheduled (basic and relatively infrequent) maintenance is performed, GW reuse can be safe to the environment and human health.

Potential impacts of on-site greywater reuse in landscape irrigation.

This study investigated the effects of irrigation with different types of waters on soil, plants, and public health. The test plant was ryegrass grown in 12 planters filled with sandy loam soil and irrigated with three types of waters (4 planters for each type): freshwater, raw domestic light greywater (GW), and treated domestic light GW. The sodium adsorption ratio (SAR), EC, pH and alkalinity of the three types of irrigation waters did not differ significantly, suggesting that raw or treated light GW should not exhibit negative effects. Concentrations of anionic and cationic surfactants in the freshwater and the treated GW were about the same, while their concentrations in the raw GW were higher. Surfactant levels in the three drainage water types were low. Some accumulation of surfactants occurred in planters irrigated with raw and treated GW. The COD of the drainage water of planters irrigated with raw GW was higher than the COD of other two drainage water types. Although raw and treated GW contained faecal coliforms, they were hardly detected in the drainage waters. All plants did not show any signs of stress. This may be due to the fact that the GW originated mainly from showers and washbasins.